Abstract

Chlorantraniliprole is an insecticide that has been widely used in agriculture and has been found to be toxic to aquatic organisms as well as in in vitro studies. The present study evaluates the invitro and invivo cytotoxicity of chlorantranilitprole in selected models. The cytotoxic potential of chlorantraniliprole was examined invitro using MTT reduction assay at concentrations of 18.5, 9.25, 4.62, 2.3, 1.15, 0.57, 0.28, 0.14 mg/mL. The IC50 values of the compound was calculated by plotting the concentration against per cent cell inhibition using Graph pad prism v.5.0. The IC50 value obtained in HepG2 cell line was found to be 1.517 mg/mL. The invivo test was conducted by Brine Shrimp (Artemia salina) mortality assay at concentrations of 500,100, 50, 25,and 10, 5mg/mL for 24 hours. After the treatment, the number of live, mobile and deceased shrimp were recorded. TheLC50 value of Chlorantraniliprole against Artemia was found to be 2.228 mg/mL (95% confidence interval
range 1.714 to 2.918). The findings of this study demonstrate that Chlorantraniliprole 18.5 SC can adversely affect aquatic arthropods, irrespective of their concentrations as well as effecting cytotoxicity in mammalian cells.

Keywords : Chlorantraniliprole 18.5 SC, HepG2 cells, MTT assay, Artemia