Abstract

This study aimed to develop an eco-friendly mycological staining reagent using red pigments extracted from beetroot (Beta vulgaris L.) pomace. Beetroot, rich in water-soluble betalains, was subjected to a blending-based extraction method, which enhanced pigment release, accelerated extraction by increasing surface area, and preserved pigment integrity. The extract was subsequently filtered through Whatman filter paper and stabilized using seventy percent ethanol to enhance colour intensity, improved pigment preservation, and extend stain shelf life. The efficacy of the novel stain was evaluated for the microscopic examination of skin impression smears from dogs suspected of fungal infections. The reagent provided clear visualization of the characteristic morphological structures of Malassezia pachydermatis, demonstrating its reliability and safety as a diagnostic stain. This plant-based, non-toxic staining technique offers a sustainable alternative to conventional chemical stains, aligning with the principles of environmental sustainability while ensuring effective fungal identification.

Keywords : Yeast, Malassezia, Dog, Beet root extract